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[Preprint]. 2023 Feb 2:2023.01.30.526315. [Version 1] doi: 10.1101/2023.01.30.526315

Figure 7: Reactivity with wt and rsVLPs by ELISA.

Figure 7:

Sera were collected from mice immunised twice with PBS or 100 AgU of EVA71 particles in the presence or absence of adjuvant (adj), indicated along the x-axis. Serum samples were then tested for reactivity with (A) wtVLPs or (B) rsVLPs. To each well, approximately 200 AgU of VLP was added, and sera were assessed at dilutions between 1:250 and 1:8000 in duplicate. Samples were considered positive if the average OD was >2x that of the negative (no serum) control wells within each plate. Graphs show the endpoint titre for individual animals, mean ± SEM, n = 8 in duplicate (n = 7 for PBS adj+ group). Abbreviations: adj-; unadjuvanted immunogen, adj+; adjuvanted immunogen, virus; genotype B2 virus immunogen, 18/116; genogroup C4 virus immunogen.