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[Preprint]. 2023 Oct 19:2023.02.01.526536. Originally published 2023 Feb 1. [Version 3] doi: 10.1101/2023.02.01.526536

Fig. 1. Comparison of modification reaction kinetics for authentic tRNA transcripts and tRNAs embedded within 5’- and 3’-end hairpins.

Fig. 1.

A, Structure and sequence of the in vitro transcription template containing 5’- and 3’-hairpins on each side of the tRNA. The RT primer binding site in the 3’-region is also indicated B, Single-turnover reaction plots for authentic tRNA transcripts (wild-type; circles) and SHAPE tRNAs (5’/3’-linkers; squares) for tRNAGly-GCC (left), tRNATrp-CCA (middle), and tRNAVal-UAC (right).