Fig. 3. Trm10 induces specific conformational changes in substrate tRNAs that are not observed in nonsubstrate tRNAs.
A, Schematic of comparison being made between free tRNA and tRNA bound to wild-type Trm10. B, Difference SHAPE reactivities between Trm10-bound and free tRNAs mapped onto secondary (top) and tertiary (bottom) structures for (left to right): tRNAGly-GCC (substrate), tRNATrp-CCA (substrate), tRNAVal-UAC (partial substrate), and tRNALeu-CAA (nonsubstrate), and tRNASer-UGA (nonsubstrate). The color scale for difference in SHAPE reactivity (Trm10-bound minus free tRNA) is shown in the bottom left.
