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[Preprint]. 2023 Oct 19:2023.02.01.526536. Originally published 2023 Feb 1. [Version 3] doi: 10.1101/2023.02.01.526536

Fig. 3. Trm10 induces specific conformational changes in substrate tRNAs that are not observed in nonsubstrate tRNAs.

Fig. 3.

A, Schematic of comparison being made between free tRNA and tRNA bound to wild-type Trm10. B, Difference SHAPE reactivities between Trm10-bound and free tRNAs mapped onto secondary (top) and tertiary (bottom) structures for (left to right): tRNAGly-GCC (substrate), tRNATrp-CCA (substrate), tRNAVal-UAC (partial substrate), and tRNALeu-CAA (nonsubstrate), and tRNASer-UGA (nonsubstrate). The color scale for difference in SHAPE reactivity (Trm10-bound minus free tRNA) is shown in the bottom left.