Table 1.

Binding interaction summary of PlzA_WT and the tested nucleic acids at various c-di-GMP concentrations.

glpFKD(−7/+35) dsDNA
c-di-GMP (μM)	Kd(app) (μM), 95% C.I. [LL, UL]	Bmax (% Shifted), 95% C.I. [LL, UL]	Adj. R-squared
100	1.09, [0.97, 1.23]	105.20, [102.20, 108.40]	0.986
50	1.25, [1.08, 1.45]	105.50, [101.50, 109.90]	0.981
25	1.33, [1.10, 1.59]	101.60, [96.58, 107.10]	0.971
10	2.81, [1.98, 4.02]	113.00, [98.79, 132.30]	0.940
Equimolar	4.22, [3.40, 5.26]	115.40, [104.40, 129.10]	0.983
0	7.86, [4.85, 14.06]	94.25, [71.72, 138.80]	0.950
glpFKD(UTR) RNA
c-di-GMP (μM)	Kd(app) (μM), 95% C.I. [LL, UL]	Bmax (% Shifted), 95% C.I. [LL, UL]	Adj. R-squared
100	0.95, [0.73, 1.22]	102.6, [96.76, 109.20]	0.930
Equimolar	4.14, [2.79, 6.30]	106.7, [89.50, 132.60]	0.944
0	7.64, [4.71, 13.66]	87.73, [66.88, 128.80]	0.950
glpF ssDNA
c-di-GMP (μM)	Kd(app) (μM), 95% C.I. [LL, UL]	Bmax (% Shifted), 95% C.I. [LL, UL]	Adj. R-squared
100	4.42, [2.94, 6.84]	102.80, [85.32, 129.90]	0.920
0	8.74, [5.67, 14.70]	82.04, [63.53, 116.90]	0.950