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[Preprint]. 2024 Jan 22:2023.01.30.526351. Originally published 2023 Jan 31. [Version 4] doi: 10.1101/2023.01.30.526351

Table 2.

Oligonucleotides used in this study.

Target Name and Sequence (5’ ➔ 3’) Size (bp)
Cloning and Sequencing Primers
plzA plzA F:
GGCAGCCATATGCTTTTATCTAGAAAAATAAGAGATTATG
plzA R:
GTGGTGCTCGAGTTAATTGAAATAATCATGGATCAAC		 786
MCS pET28a(+):plzA T7 F:
TAATACGACTCACTATAGGGT7
R:
TAGTTATTGCTCAGCGGTGG		 1026
pCR 2.1 TA clones M13 F:
GTAAAACGACGGCCAG
M13 R:
CAGGAAACAGCTATGAC		 N/A
plzA-NTD plzA 1F:
ATGCTTTTATCTAGAAAAATAAGAGATTATG
plzA N R:
TTACTGATTTTGCCCAAGCTTTAAATC		 435
plzA-CTD plzA C F:
ATGGATTTAAAGCTTGGGCAA
plzA 1R:
ATTGAAATAATCATGGATCAACATAGTATAC		 378
Site-directed Mutagenesis Primers
plzA R145-149D plzA-mut F:
AATCAGGACATTCATGAGGACATTATTATCGATAAAGATTCTATTAG
plzA-mut R:
GTCCTCATGAATGTCCTGATTTTGCCCAAGCTTTAAATCAAGAAGCTTTCC		 786
EMSA Probes, Competitors, and Primers
glpFKD(−219) M13 F:
GTAAAACGACGGCCAG
M13 R 5’IRD800CW or unlabeled:
CAGGAAACAGCTATGAC		 654
glpFKD(−7/+35) glpF F IRD800:
ATTAAATATAATTTTAATAAGGCTTTTATTAGAAAAATTAAT
glpF R:
ATTAATTTTTCTAATAAAAGCCTTATTAAAATTATATTTAAT		 42
ssglpF glpF F IRD800:
ATTAAATATAATTTTAATAAGGCTTTTATTAGAAAAATTAAT		 42
glpFKD(UTR) RNA glpF RNA 5’ Alexa488:
AUAAUUUUAAUAAGGCUUUUAUUAGAAAAAUUAAU		 35
pCR 2.1 competitor M13 F:
GTAAAACGACGGCCAG
M13 R:
CAGGAAACAGCTATGAC		 210
a

Bolded and underlined nucleotides indicate restriction enzyme sites.