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[Preprint]. 2023 Jun 15:2023.02.01.526652. [Version 4] doi: 10.1101/2023.02.01.526652

Figure 5. Only CrxE80A/+ retinas maintain photoreceptor OS and residual rod ERG response.

Figure 5.

(A-E) Hematoxylin-eosin (H&E) staining of P21 retina sections show that photoreceptor OS layer is absent in all mutant retinas except CrxE80A/+. OS: outer segment; ONL: outer nuclear layer; INL: inner nuclear layer; IPL: inner plexiform layer; GCL: ganglion cell layer. Scale bar, 100μm. (F-J) Rhodopsin (RHO, red) immunostaining is present in CrxE80A/+, CrxE80A/A, and CrxK88N/+ retinas and absent in CrxK88N/N retina. Cone arrestin (mCAR, green) immunostaining is absent in all mutant retinas. Nuclei were visualized by DAPI staining (Blue). Scale bar, 100μm. (K-M) The electroretinogram responses (ERG) recorded from 1-month mice. Error bars represent the standard error of the mean (SEM, n ≥ 4). p-value: Two-way ANOVA and Tukey’s multiple comparisons. ****: p ≤ 0.0001. ns: >0.05.