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[Preprint]. 2023 Jan 31:2023.01.30.526383. [Version 1] doi: 10.1101/2023.01.30.526383

Fig. 2. Catalytic mutant and endogenous A3B are relocalized by HCMV.

Fig. 2.

(A-E) Representative IF microscopy images of the indicated cell types stably expressing A3B-E255A-HA incubated with medium alone (mock) or infected with the indicated HCVM strains for 72 hrs (10 μm scale).

(F) Quantification A3B-E255A-HA subcellular localization phenotypes shown in panels A-E. Each histogram bar reports the percentage of cells with cytoplasmic A3B-HA (n>100 cells per condition; mean +/− SD with indicated p-values from unpaired student’s t-tests).

(G) Representative IF microscopy images of ARPE19 cells incubated with medium alone (mock) or infected with TB40-mCherry for 72 hrs, stained for endogenous A3B (10 μm scale).

(H) Quantification of endogenous A3B subcellular localization phenotype shown in panel G. The dot-plot chart shows the ratio between nuclear and cytoplasmic fluorescence intensity (n>50 cells per condition; p-values were obtained using unpaired student’s t-tests).