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[Preprint]. 2023 Jan 31:2023.01.30.526383. [Version 1] doi: 10.1101/2023.01.30.526383

Fig.3. A3B relocalization is UL45-independent.

Fig.3.

(A) Coimmunoprecipitation of transfected HCMV UL45-FLAG with the indicated A3-HA constructs in HEK293T cells. Cells co-transfected with EBV BORF2 and A3B or A3G are used as positive and negative controls, respectively.

(B) TBE-urea PAGE analysis of A3B deaminase activity in the presence of empty vector, HCMV UL45, or EBV BORF2.

(C) Representative IF microscopy images of HeLa cells transiently expressing A3B-HA together with empty vector, HCMV UL45-FLAG or EBV BORF2-FLAG (10 μm scale).

(D-E) Representative IF microscopy images of the indicated cell types stably expressing A3B-HA incubated with medium alone (mock) or infected with the indicated HCVM strains and UL45-null derivatives for 72 hrs (10 μm scale).