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[Preprint]. 2023 Jan 31:rs.3.rs-2460097. [Version 1] doi: 10.21203/rs.3.rs-2460097/v1

Fig. 4. Bacteroides ovatus improved GVHD-related mortality in meropenem-aggravated colonic GVHD via suppressing the abundance of B. theta.

Fig. 4.

(A) Experimental schema of murine GVHD model using meropenem treatment followed by oral gavage of 20 million colony-forming units of B. ovatus daily for 3 days. (B) Overall survival after allo-HSCT. Data are combined from two independent experiments. (C) Bacterial densities of mouse stool samples collected on day 21 after administering meropenem by drinking water. Bacterial densities were measured by 16S rRNA gene qPCR. (D) Alpha diversity, measured by the Shannon index, was quantified in fecal samples. (E) Bacterial genera composition of fecal samples. (F) Relative abundance of B. ovatus (left) and B. theta (right). (G) Periodic acid-Schiff (PAS) staining of histological colon sections collected on day 23. Bar, 100 μm. The areas inside dotted lines indicate the inner dense colonic mucus layer. (H) Mucus thickness on day 23. Data are shown from one representative experiment.