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[Preprint]. 2023 Jan 31:rs.3.rs-2460097. [Version 1] doi: 10.21203/rs.3.rs-2460097/v1

Extended Data Fig. 3. Introduction of Bacteroides ovatus did not alter abundance and functionality of B. theta in meropenem-untreated allo-HSCT mice.

Extended Data Fig. 3.

(A) Experimental schema of murine GVHD model with oral gavage of 20 million colony-forming units of B. ovatus daily from days 16 to 18. (B) Overall survival after allo-HSCT. Data are combined from two independent experiments. (C) Bacterial densities of mouse stool samples collected on day 21. Bacterial densities were measured by 16S rRNA gene qPCR. (D) Alpha diversity, measured by the Shannon index, was quantified in fecal samples. (E) Bacterial genera composition of fecal samples. (F) Relative abundance of B. ovatus (left) and B. theta (right). (B-F) Combined data from two independent experiments. (G) PAS staining of histological colon sections collected on day 23. Bar, 100 μm. The areas inside dotted lines indicate the inner dense colonic mucus layer. (H) Mucus thickness on day 23. Data are shown from one representative experiment.