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. 2023 Jan 17;24(3):1844. doi: 10.3390/ijms24031844

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Differentially accumulated metabolite (DAM) analysis of the metabolome. Heatmaps of DAMs in D1-Sp vs. D2-Sp (A), D1-Sp vs. D3-Sp (B), and D1-Sp vs. D4-Sp (C). (D) Venn analysis of D1-Sp vs. D2-Sp, D1-Sp vs. D3-Sp, and D1-Sp vs. D4-Sp. The color scale from Min (blue) to Max (red) indicates the metabolite contents from low to high. Identification of DAMs between three comparison groups was performed using variable importance in projection values ≥1 and fold change ≥2 or ≤0.5; in addition, the content must be ≥ 1 μg/g DW in spots in at least one stage.