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. 2022 Aug 19;164(3):625–637. doi: 10.1097/j.pain.0000000000002750

Figure 4.

Figure 4.

Optosilencing of LSV CaMKIIα-positive neurons attenuates visceral hyperalgesia, whereas optoactivation of LSV CaMKIIα-positive neurons promotes adaptive visceral pain behavior. (A) Experimental procedure of optogenetic manipulation of LSV CaMKIIα-positive neurons (left) and schematic of EMG recording for evaluating visceral pain (right). (B) Representative image of viral expression in LSV CaMKIIα-positive neurons (left) and summary view of fiber tip and injection site (right). Black dots and green dots indicate the fiber tip and injection site, respectively. Scale bar, 200 μm. (C) The representative EMG traces of NMD mice were recorded at 20, 40, 60, and 80 mm Hg, respectively. (D) The area under the curve of the EMG at 20, 40, 60, and 80 mm Hg in NMD mice (F (3, 15) = 121.3, ***P < 0.001, two-way repeated-measure ANOVA followed by Bonferroni post hoc test, n = 6 mice per group). (E) Representative EMG traces of CON mice were recorded at 20, 40, 60, and 80 mm Hg, respectively. (F) The area under the curve of EMG at 20, 40, 60, and 80 mm Hg in CON mice (F (3, 15) = 117.0, ***P < 0.001, two-way repeated-measure ANOVA followed by Bonferroni post hoc test, n = 6 mice per group). White dotted frame indicates the optical fiber trace. n.s. indicates nonsignificant differences, P > 0.05. See also Figure S3, available at http://links.lww.com/PAIN/B695. ANOVA, analysis of variance; CRD, colorectal distention; EMG, electromyography; LSV, lateral septal ventral; NMD, neonatal maternal deprivation.