Radium-223 and enzalutamide combination treatment does not affect bone microarchitecture. The mice (n = 9/group) were treated with vehicle, radium-223 (330 kBq/kg, Q4W × 2, i.v.), enzalutamide (30 mg/kg, QD, p.o.), or their combination. Bone structure parameters (relative to vehicle) described as (A) total bone volume (BV), (B) trabecular bone volume fraction (BV/TV), (C) trabecular thickness, (D) trabecular separation, (E) trabecular number, (F) cortical cross-sectional bone area, (G) cortical thickness, and (H) cortical bone specific surface (BS/BV). Plots indicate mean and 95% confidence interval (CI). The statistical analyses were performed using ANOVA followed by contrasts or Kruskal–Wallis test followed by Dunn’s test: * p < 0.05; ** p < 0.01; *** p < 0.001.