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Post-embedding von Kossa staining of nuclear chromatin in mineralizing AVICs. Major silver particle precipitation onto nuclear heterochromatin (rectangular boxes) in mineralizing AVICs from (A) 28-day-long subdermally implanted pAVLs, with the largest silver particles being distributed along peripheral PPLs (counterposed arrowheads), (B) stenotic hAVLs, and (C) pro-calcific cultures. Bar: 0.25 μm.
