Figure 5.

Schematic diagram of the design strategies adopted in the fabrication of microfluidic chip-derived in vitro BBB models (A) Sandwich Design: Upper and lower PDMS channel separated by a membrane. The apical channel is plated with endothelial cells, whereas the basolateral channel consists of astrocytes and/or pericytes. (B) Parallel Design: This consists of two parallel horizontal channels with perforations in the inner channel that provide contact between them. The outer channel has endothelial cells, whereas inner channel consists of astrocytes or pericytes dispersed in matrix. (C) 3D Tubular Structure Design: This consists of central cylindrical channel in collagen matrix. The central cylindrical channel has endothelial cells, whereas the matrix has astrocytes or pericytes dispersed in matrix. (D) Vasculogenesis Design: This consists of the central channel having endothelial cells, astrocytes, or pericytes dispersed in matrix flanked by two channels nourishing the media.