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. 2023 Jan 19;24(3):2023. doi: 10.3390/ijms24032023

Figure 6.

Figure 6

The UPR branch Perk is activated when co-treating cells with 250 µM BPA and 250 µM TUDCA. (A) MIN6 cells were treated for 8 h with BPA, TUDCA, DMSO and 5 µg/m Tm. Whole-cell lysates were analyzed by Western blot for Bip, Atf4, P-eIF2α, total eIF2α, Chop and Vinculin. (B) Graphs depict quantifications of the immunoblots. Vinculin was used as a normalizer. * p < 0.05, ** p < 0.005, compared to vehicle; %%%% p < 0.0001 compared to DMSO; • p < 0.05, •• p < 0.005 compared to TUDCA, based on One way ANOVA. Graphs represent mean ± SD.