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. 2023 Feb 10;18(2):e0277478. doi: 10.1371/journal.pone.0277478

Fig 4. Clear native PAGE analysis of the RBBP6-p53BD.

Fig 4

Clear native PAGE analysis of purified RBBP6-p53BD. (A): Tris-HCl buffer, with pH 8.3. Lane 1 is the RBBP6-p53BD in 50 mM sodium phosphate, pH 7. Lane 2 is the RBBP6-p53BD in 50 mM sodium phosphate, pH 7 after being heated to and cooled from 90°C to 20°C, as described in section 2.8. Lane 3 is the RBBP6-p53BD in 50 mM sodium phosphate, pH 7 with 2 M urea. (B): Imidazole-HEPES buffer, pH7.4. Lane 1 is the RBBP6-p53BD in 50 mM sodium phosphate, pH 7, and Lane 2 is the RBBP6-p53BD in 50 mM sodium phosphate, pH 7 after being heated to and cooled from 90°C to 20°C, as described in section 2.8. Protein samples were not equalised before loading onto the gels.