Figure 6.

Analysis of the expression of neurog1 and neurod1 neural markers by whole-mount in situ hybridization (WISH) after treatment of zebrafish embryos with cabotegravir (CAB) by the immersion method. Representative images of WISH analyses of neurog1 and neurod1 expression in embryos treated with CAB by the immersion method. CAB was dissolved in fish water containing 0.1% dimethyl sulfoxide (DMSO). Fish water with 0.1% DMSO was used as negative control [CO]. (A) Ventral and dorsal views are shown for neurog1 expression at 16 h post fertilization (hpf); (B) lateral and dorsal views are shown for neurod1 expression at 96 hpf. (C,D) Percentages of embryos with defects. Experiments were performed twice with 25 embryos for each experimental point. (** p < 0.005 vs. control group). d, diencephalon; mhb, midbrain hindbrain boundary; cg, cranio facial ganglia; t, telencephalon.