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. 2023 Jan 31;24(3):2648. doi: 10.3390/ijms24032648

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Flow cytometric analysis of A549 cells treated with IC₅₀/2 and IC₅₀ concentrations of compounds 3b, 4a, and cisplatin. At least 10,000 cells were analyzed per sample, and quadrant analysis was performed. Q1-LR, Q1-UR, Q1-LL, and Q1-UL quadrants represent early apoptosis, late apoptosis, viability, and necrosis, respectively. (a) Control; (b) Control; (c) Compound 3b at IC₅₀/2 concentration; (d) Compound 3b at IC₅₀ concentration; (e) Compound 4a at IC₅₀/4 concentration; (f) Compound 4a at IC₅₀/2 concentration; (g) Cisplatin at IC₅₀/2 concentration; (h) Cisplatin at IC₅₀ concentration.

Flow cytometric analysis of A549 cells treated with IC₅₀/2 and IC₅₀ concentrations of compounds 3b, 4a, and cisplatin. At least 10,000 cells were analyzed per sample, and quadrant analysis was performed. Q1-LR, Q1-UR, Q1-LL, and Q1-UL quadrants represent early apoptosis, late apoptosis, viability, and necrosis, respectively. (a) Control; (b) Control; (c) Compound 3b at IC₅₀/2 concentration; (d) Compound 3b at IC₅₀ concentration; (e) Compound 4a at IC₅₀/4 concentration; (f) Compound 4a at IC₅₀/2 concentration; (g) Cisplatin at IC₅₀/2 concentration; (h) Cisplatin at IC₅₀ concentration.