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. 2023 Jan 19;24(3):1987. doi: 10.3390/ijms24031987

Figure 2.

Figure 2

Separation of leucine (m/z 132.102), isoleucine (m/z 132.102), and arginine (m/z 175.120) in human serum extracts using an ACQUITY UPLC BEH Amide column (50 mm × 2.1 mm i.d.; 1.7 μm particle size) coupled to an ACQUITY UPLC BEH Amide VanGuard pre-column (5 mm × 2.1 mm i.d.; 1.7 μm particle size) under seven different mobile phase modifiers. The data were acquired in ESI(+). The red arrows indicate a drop in the signal due to the coelution of highly abundant metabolites or mobile phase impurities. Legend: see Figure 1.