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. 2023 Jan 19;24(3):1987. doi: 10.3390/ijms24031987

Figure 3.

Figure 3

Separation of hexose phosphates (m/z 259.022) in the cell extracts on an ACQUITY UPLC BEH Amide column (50 mm × 2.1 mm i.d.; 1.7 μm particle size) coupled to an ACQUITY UPLC BEH Amide VanGuard pre-column (5 mm × 2.1 mm i.d.; 1.7 μm particle size) using a mobile phase with seven mobile phase modifiers in ESI(−). The peaks were annotated using authentic standards, including fructose 6-phosphate (4.8 min), glucose 1-phosphate/mannose 6-phosphate/galactose 6-phosphate (4.88 min; individual standards were not separated under the conditions), and glucose 6-phosphate (4.97 min) under the conditions of 10 mM ammonium formate and 0.125% formic acid used as the mobile phase modifiers. Legend: see Figure 1.