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. 2023 Jan 28;24(3):2511. doi: 10.3390/ijms24032511

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Location of primers in the potato leafroll virus (PLRV) coat protein gene. Free web-based Bio-edit software (https://www.bioinformatics.nl/cgi-bin/primer3plus/primer3plus.cgi/ (accessed on 30 June 2021)) was used to run a ClustalW multiple sequence alignment. The present study followed the manufacturer’s instructions, and primers in triplicate were generated using a full-coat protein gene by specifically targeting conserved regions. NCBI’s GenBank database (http://www.ncbi.nlm.nih.gov/blast (accessed on 30 June 2021)) provided the sequences (Accessions AF453388, EU717546, AY307123, X77324, JQ420903, LC501445, FJ481109, MK445319, MH937415, MF062487, KR051205, KP090166, EF063711, D13954, KC456052, MG356502, KU586454 and GU256062). The boxes denote primer regions, while arrows have been placed to specify the primer positions for reverse transcription-recombinase polymerase amplification (RT-RPA).