Figure 2.

Illustrative model depicting the potato leafroll virus (PLRV) dissemination, symptoms of severe infection in known samples, and subsequent evaluation of virus by reverse transcription-polymerase chain reaction (RT-PCR) via newly designed primer sets in this investigation. Under current experimentation, PLRV symptomatic leaf samples were procured from a pure virus culture maintenance facility at ICAR-Central Potato Research Institute (ICAR-CPRI), Shimla, Himachal Pradesh, India. The cDNA synthesis was executed with 2 μL of 200–400 ng/μL total RNA with subsequent RT-PCR at 58 °C. The gel documentation-generated image denotes standard 100 bp ladder (Lane M), three primers set (LRRPAF1/R1: Lane 1, LRRPAF2/R2: Lane 2 and LRRPAF3/R3: Lane 3), along with the healthy control (Lane 4). The expected amplified products (bp) of 246 bp, 249 bp and 153 bp are also denoted in each lane for respective primers.