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. 2023 Jan 28;24(3):2511. doi: 10.3390/ijms24032511

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Primer optimization at different temperature regimes for two-step reverse transcription-recombinase polymerase amplification (RT-RPA) detection in potato leafroll virus (PLRV) infected samples. A total of 18 lanes depicted the respective target product amplification employing forward and reverse primers, viz., LRRPAF1/R1, LRRPAF2/R2 and LRRPAF3/R3. The primers sets are shown over each lane along with the corresponding expected amplified product. A 100 bp ladder (Lane M) along with a healthy control (Lanes 13, 14 and 15) and a negative (water) control (lanes 16, 17 and 18) are also included for the accuracy of the results.