Figure 4.

Optimization of one-step reverse transcription-recombinase polymerase amplification (RT-RPA) for potato leafroll virus (PLRV) detection using primer pair C (LRRPAF3/R3) at different temperature regimes (38, 40 and 42 °C). The gel-electrophoresis generated images depict the RT-RPA amplified amplicons in a heating block (a,c) and in a water bath (b,d). The purified RNA as the template was used in RT-RPA in (a,b); whereas the simple RNA has been used for (c,d). Within each gel documentation image, three lanes (1, 2 and 3) depict the PLRV-infected samples along with a 50 bp ladder (Lane M). Lane 5 (c,d) denotes the healthy control.