Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jan 28;24(3):2511. doi: 10.3390/ijms24032511

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Reverse transcription-recombinase polymerase amplification (RT-RPA) optimization for potato leafroll virus (PLRV) detection using forward and reverse primer LRRPAF3/R3-Set C. The RT-RPA amplified products on (a) a PCR machine and (b) a water bath using a cDNA template at a temperature of 40 °C and a duration range of 10, 20, 30, 40 and 50 min. Likewise, the RT-RPA amplified amplicons using total RNA (1 μL) in (c) a PCR machine and (d) a water bath under the same conditions of temperature and duration. Optimization of magnesium acetate concentrations ranging from 12 mM to 20 mM under a similar setup of (e) thermal cycler and (f) water bath. Lane M: 50 bp ladder; HC: healthy control.