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. 2023 Jan 28;24(3):2511. doi: 10.3390/ijms24032511

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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One-step reverse transcription-recombinase polymerase amplification (RT-RPA) optimization for potato leafroll virus (PLRV) detection using forward and reverse primer LRRPAF3/R3-Set C. Utilization of two RNA isolation methods, viz., cellular disc paper-technique [28] and Spectrum^TM Plant Total RNA kit (Sigma-Aldrich, St. Louis, MO, USA). The agarose gel images visualize the amplicons when the respective concentration of sodium sulphite ranging from 0.25 to 1.0% for the respective method of RNA extraction has been used. HC: healthy potato mini tuber sample, NC: negative control, Lane M: 50 bp marker.