Figure 7.

Reverse transcription-polymerase chain reaction (RT-PCR) and reverse transcription-recombinase polymerase amplification (RT-RPA) amplified amplicons at dilutions levels ranging from 10⁻⁰ to 10⁻⁸ detect potato leafroll virus (PLRV). One-step RT-PCR with previously reported primers [12] on purified RNA performed with (a) infected leaves and (b) infected tubers. One-step RT-PCR with optimized primer LRRPAF3/R3 on purified RNA in (c) infected leaves and (d) infected tubers. One-step RT-RPA with newly optimized primer LRRPAF3/R3 on purified total RNA in (e) infected leaves and (f) infected tubers. One-step RT-RPA with optimized primer LRRPAF3/R3 on cellular disc-mediated RNA extract in (g) infected leaves and (h) infected tubers. Lane M (a–h): 50 bp DNA ladder.