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. 2023 Jan 28;24(3):2511. doi: 10.3390/ijms24032511

Figure 8.

Figure 8

Validation of reverse-transcription recombinase polymerase amplification (RT-RPA) and reverse transcription-polymerase chain reaction (RT-PCR) in one step for potato leafroll virus (PLRV) detection (leaves). (a) One-step RT-PCR performed with previously reported primers [12] on purified RNA; (b) one-step RT-RPA with newly optimized primer LRRPAF3/R3 on purified total RNA; (c) one-step RT-RPA with optimized primer LRRPAF3/R3 on cellular disc-mediated RNA extract. Lane M (a–c): 50 bp DNA ladder, lanes 1–15 show potato leaf samples, PC: known positive, HC: healthy, NC: negative (water).