Table 1.

Primers utilized in this research study with the aim of optimizing a one-step reverse transcription-recombinase polymerase amplification (RT-RPA) technique.

Target Virus	Primer Set No.	Primers	Polarity	Sequence 5′–3′	Bases	Target Position on Coat Protein Gene	Position on Accession Number in NCBI GeneBank (NC_001747.1)	GC (%)	Tm (°C)	Amplicon Size (bp)
PLRV	A	LRRPAF1	Sense	GTATAAGATCACAAGCATCTTACTTCAGTTC	31	327–357	4019–4267	35.5	61.3	249
		LRRPAR1	Antisense	GTATCTGAAGATTTTCCATTTCCCTTC	27	548–575		37	62.5
	B	LRRPAF2	Sense	TAAGATCACAAGCATCTTACTTCAGTTC	28	330–357	4022–4267	35.7	60.5	246
		LRRPAR2	Antisense	GTATCTGAAGATTTTCCATTTCCCTTC	27	548–575		37	62.5
	C	LRRPAF3	Sense	ACTCAAGGCCTACCATGAGTATAAGAT	27	309–335	4001–4183	40.7	60.9	153 *
		LRRPAR3	Antisense	GTAATTTGGAACTTGTTGACGTAGGACT	28	434–461		39.3	63

* The best selected for optimization and validation of one-step RT-RPA.