Table 2.

Comparison and validation of potato leafroll virus (PLRV) detection methods, viz., one-step reverse-transcription recombinase polymerase amplification (RT-RPA) and reverse transcription-polymerase chain reaction (RT-PCR) of field-collected suspected plant parts of widely cultivated and popular potato cultivars across different agroecological zones in various states of India.

Field Sample No.	One-Step RT-PCR [12]	One Step RT-RPA with Primer Pair C (LRRPAF3/R3) under Heating Block/Water Bath	One Step RT-RPA with Primer Pair C (LRRPAF3/R3) under Heating Block/Water Bath
Leaves	Purified RNA	Purified RNA	Crude RNA (Cellular Disc-Mediated RNA Extract with Added 1.0% Sodium Sulphite)
1	+	+	+
2	+	+	+
3	+	+	−
4	−	−	−
5	+	+	+
6	+	+	+
7	+	+	+
8	+	+	+
9	+	+	+
10	+	+	+
11	−	−	−
12	+	+	+
13	+	+	+
14	+	+	+
15	−	−	−
Healthy Control	−	−	−
Negative Control	−	−	−
Positive Control	+	+	+
Field Sample No.	One-step RT-PCR [12]	One-step RT-RPA with primer pair C (LRRPAF3/R3) under thermal Cycler	One step RT-RPA with primer pair C (LRRPAF3/R3) under heating block/water bath
Tubers	Purified RNA	Purified RNA	Purified RNA
1	+	+	+
2	+	+	+
3	+	+	+
4	+	+	−
5	−	−	−
6	+	+	+
7	+	+	+
8	+	+	+
9	+	+	+
10	+	+	+
Healthy Control	−	−	−
Positive Control	+	+	+

(+): PLRV positive; (−): PLRV negative.