Figure 4.

Impact of TLR4 agonists present on GMMA on the polysaccharide-specific humoral response elicited by Shigella GMMA-based vaccine. (a) LPS-specific (S. sonnei) or OAg-specific (S. flexneri) total IgG (EU/mL) after boost observed in wild type (WT) and TLR4 mutant mice (TLR4^mut). (b) Bactericidal titers expressed as IC₅₀. Mice were immunized intramuscularly at days 0 and 28 with the quadrivalent GMMA-based vaccine against Shigella: 9.4 ng OAg of each one of the four GMMA have been administered to each mouse, either in absence or presence of 0.7 mg/mL of Alhydrogel Al³⁺. Geometric mean (bar) is reported for all groups together with individual values (dots) and 95% confidence intervals (CI). Responses elicited in WT and TLR4^mut mice by each GMMA were compared by Mann–Whitney test; * p < 0.05; ** p < 0.01; *** p < 0.001.