Figure 5.

Impact of TLR4 and TLR2 agonists present on GMMA on the GMMA proteins-specific humoral response elicited by Shigella GMMA-based vaccine. (a) Shigella GMMA proteins-specific total IgG (EU/mL) after boost observed in wild type and TLR4 mutant (TLR4^mut) mice and in (b) wild type and TLR2 deficient (TLR2^−/−) mice. Mice were immunized intramuscularly at days 0 and 28 with the quadrivalent GMMA-based vaccine against Shigella: 9.4 ng OAg of each one of the four GMMA have been administered to each mouse, either in absence or presence of 0.7 mg/mL of Alhydrogel (Al³⁺). Geometric mean (bar) is reported for all groups together with individual values (dots) and 95% confidence intervals (CI). No statistically significant difference was observed between WT and TLR4^mut or WT and TLR2^−/− mice using Mann–Whitney test.