Figure 6.

Impact of TLR2 agonists present on GMMA on the polysaccharide-specific humoral response elicited by Shigella GMMA-based vaccine. (a) LPS-specific (S. sonnei) or Oag-specific (S. flexneri) total IgG (EU/mL) after boost observed in wild type (WT) and TLR2 deficient mice (TLR2^−/−). (b) Bactericidal titers expressed as IC₅₀. Mice were immunized intramuscularly at days 0 and 28 with the quadrivalent GMMA-based vaccine against Shigella: 9.4 ng (quantified as total Oag) of each one of the four GMMA have been administered to each mouse, either in absence or presence of 0.7 mg/mL of Alhydrogel. Geometric mean (bar) is reported for all groups together with individual values (dots) and 95% confidence intervals (CI). No statistically significant difference was observed between WT and TLR2^−/− mice using Mann–Whitney test.