Figure 5.

Quantification of CA1 neuron numbers and new-born neurons in the dentate gyrus (DG). (a) Caffeine treatment resulted in statistically significant higher CA1 neuron numbers in Tg4-42 mice housed under SH conditions, however, no effect of caffeine was detected under EE conditions. (b) Caffeine-treated Tg4-42 mice had increased numbers of DCX-positive neurons compared to vehicle-treated littermates. No additional effect of caffeine was observed in EE-housed mice (b). Two-way ANOVA followed by Bonferroni’s multiple comparison test (a,b). ** p < 0.01. All data are given as mean ± SD. Data from vehicle- and caffeine-treated SH and vehicle-treated EE Tg4-42 groups have been partially included in previous studies [7,39] and were part of a larger set of experiments in order to minimize experimental animal numbers.