Figure 4.

Type and quality of immunostaining obtained with different antibodies in different animal species and brain regions. (A) Four types of staining were considered, including a clear and clean staining without background noise, a specific staining bleary with background, an unspecific staining (with or without background; including artifacts, or staining associated with other structures, e.g., blood vessels, astrocytes, other neurons), and the absence of signal. Bottom: legend with symbols reported in the Results Tables. (B), Some examples considering substantial differences depending on the different antibodies used. LV: lateral ventricle. Scale bars: 30 µm.