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. 2023 Jan 28;24(3):2514. doi: 10.3390/ijms24032514

Table 3.

(a) Occurrence and quality of staining for DCX. (b) Merge (with and without citrate treatment).

(a)
Citrate Treatment (5 min)
Antibodies Regions Mouse Marmoset Rabbit Cat Sheep
(perfusion)		 Sheep
(immersion)
Santa Cruz
(goat)		 pc ●
nc ●
svz ●
sgz * ●
Cell Signalling
(rabbit)		 pc * * * *
nc * * * *
svz *
sgz * * * * *
Abcam
(rabbit)		 pc * ● * * * *
nc ● * * *
svz ●
sgz * ● * * *
Santa Cruz
(mouse)		 pc * * * ●
nc * * * ●
svz * ●
sgz * * ●
Millipore
(guinea pig)		 pc * * * *
nc * * *
svz * *
sgz * * * * *
No Citrate
Antibodies Regions Mouse Marmoset Rabbit Cat Sheep
(perfusion)		 Sheep
(immersion)
Santa Cruz
(goat)		 pc * * ●
nc * * * ●
svz * ●
sgz * * * ●
Cell Signalling
(rabbit)		 pc * * * ●
nc * * * * ●
svz * * ●
sgz * * * * ●
Abcam
(rabbit)		 pc * ● * * *
nc ● * * *
svz ●
sgz ● * * * *
Santa Cruz
(mouse)		 pc * * * *
nc * * * ●
svz * * * *
sgz * * * *
Millipore
(guinea pig)		 pc * * * * *
nc * * * *
svz * * * *
sgz * ● ● * *
(b)
Antibodies Regions Mouse Marmoset Rabbit Cat Sheep
(perfusion)		 Sheep
(immersion)
Santa Cruz
(goat)		 pc (5′) (5′)
nc (5′) (5′) (5′)
svz (5′)
sgz (5′) (5′)
Cell Signalling
(rabbit)		 pc only 5′ C
nc only 5′ C
svz (5′) (5′) only 5′ C
sgz (5′) only 5′ C
Abcam
(rabbit)		 pc without C without C
nc without C
svz
sgz without C (5′)
Santa Cruz
(mouse)		 pc only 5′ C only 5′ C only 5′ C only 5′ C
nc only 5′ C only 5′ C
svz only 5′ C (5′) (5′) only 5′ C
sgz (5′) only 5′ C only 5′ C
Millipore
(guinea pig)		 pc (5′)
nc
svz (5′) (5′) without C
sgz without C

Subtable (a): Inline graphic Absence of staining due to real interspecies difference (e.g., no cell population containg DCX in the mouse neocortex); Inline graphic Clear and clean immunoreaction; Inline graphic Immunoreaction with background; Inline graphic Immunoreaction with non-specific staining; Inline graphic No signal with background; Inline graphic No signal with non-specific staining; Inline graphic No signal; Subtable (b): MERGE of results obtained with and without 5′ Citrate: Inline graphic Same results; Inline graphic Different results; (5′) better with Citrate; only (5′) C signal only with Citrate; without C signal only without Citrate.