Table 4.
(a) Occurrence and quality of staining for Ki67 antigen. (b) Merge (with and without citrate treatment).
(a) | |||||||
Citrate Treatment (5 min) | |||||||
Antibodies | Regions | Mouse | Marmoset | Rabbit | Cat |
Sheep (perfusion) |
Sheep (immersion) |
BD Pharmingen (mouse) |
svz | * | * | ||||
sgz | * | ||||||
Abcam (rabbit) |
svz | * | * | * | * | ||
sgz | * | * | * | ● | |||
No Citrate | |||||||
Antibodies | Regions | Mouse | Marmoset | Rabbit | Cat | (perfusion) | (immersion) |
BD Pharmingen (mouse) |
svz | * | * | * | * | ||
sgz | ● | * | * | ● | |||
Abcam (rabbit) |
svz | * | ● | * | * | * | |
sgz | * | * | * | ||||
(b) | |||||||
Antibodies | Regions | Mouse | Marmoset | Rabbit | Cat |
Sheep (perfusion) |
Sheep (immersion) |
BD Pharmingen (mouse) |
svz | (5′) | (5′) | only 5′ C | |||
sgz | only 5′ C | only 5′ C | only 5′ C | ||||
Abcam (rabbit) |
svz | only 5′ C | (5′) | only 5′ C | |||
sgz | only 5′ C | only 5′ C | only 5′ C |
Subtable (a): Absence of staining due to real interspecies difference (e.g., no cell population containg DCX in the mouse neocortex);
Clear and clean immunoreaction;
Immunoreaction with background;
Immunoreaction with non-specific staining;
No signal with background;
No signal with non-specific staining;
No signal; Subtable (b): MERGE of results obtained with and without 5′ Citrate:
Same results;
Different results; (5′) better with Citrate; only (5′) C signal only with Citrate; without C signal only without Citrate.