Table 6.

Brain samples and antibodies used in this study.

Brain Tissues Analysed
Species	Life Stage	Fixation	Fixative		PMI	Sample processing
Mouse	3 months	Perfusion	4% PFA		none	-Whole hemisphere cut into coronal slices (1–2 cm thick) and washed in phosphate buffer solution -Cryo-protection in sucrose solutions -Frozen by immersion in liquid nitrogen-chilled isopentane -Cut into 40 μm thick cryostat coronal sections
Marmoset	Adult	Immersion	4% PFA (15% picric acid)		1 h
Rabbit	3.5 years	Perfusion	4% PFA		none
Cat	1.5 years	Immersion	4% buffered formalin		1 h
Sheep	2 years	Perfusion	4% PFA		none
	5 years	Immersion	4% buffered formalin		20 min
Human (see Table 7)	Adult (post-mortem)	Immersion	4% buffered formalin		16–23 h
	Adult (intraoperative)		4% PFA		none	-Brain samples cut into slice (0.5 cm thick); then, processed as above
Primary Antibodies Tested
Antigen	Host	Type	Code	Raised against (source: data sheet and company information)	Dilution	Source
DCX	Goat	Polyclonal	SC8066	Epitope within the last 50 c-terminal amino acids	1:1000	Santa Cruz Biotechnology, Dallas, Texas, USA
	Mouse	Monoclonal	SC271390	Amino acids 81-365 mapping at the C-terminus of Doublecortin of human origin
	Rabbit	Polyclonal	ab18723	Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of human doublecortin		Abcam, Cambridge, UK
			4604	Antigenic sequence surrounds amino acid 350 tyrosine of human doublecortin		Cell Signalling, Danvers, Massachusetts, USA
	Guinea pig		ab2253	Epitope aminoacidic sequence: YLPLSLDDSDSLGDSM		Merck Millipore, Burlington, Massachusetts, USA
Ki67	Rabbit		ab15580	Synthetic peptide	1:500	Abcam, Cambridge, UK
	Mouse	Monoclonal	550609	Human Ki67		BD Pharmingen, San Diego, California, USA