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In vitro blood–brain barrier assay for TF2D-5 and TF2D-5a. An amount equaling 1 mg/mL TF2D-5 (A) or TF2D-5a (B) was added to an insert that was seeded and covered with endothelial and astroglial cells, representing the in vitro blood–brain barrier, or an empty insert serving as a control. After different time points, samples were taken and analyzed by quantitative HPLC analysis. Data given as mean ± SD out of three independent replicates.
