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. 2023 Jan 21;24(3):2161. doi: 10.3390/ijms24032161

Figure 12.

Figure 12

Stability assay of TF2D-5 and TF2D-5a using different simulated human body fluids. (A) Stability of compounds in simulated gastric fluid (SGF). The simulated gastric fluid consisted of 2 mg/mL sodium chloride, 3.2 mg/mL pepsin, and 80 mM HCl; pH 1. (B) Stability of TF2D-5 and TF2D-5a in simulated intestinal fluid (SIF). Simulated intestinal fluid consisted of 6.8 mg/mL potassium dihydrogen phosphate, 10 mg/mL pancreas powder, and 15.4 mM NaOH; pH 6.8. (C) Stability of TF2D-5 and TF2D-5a in human blood plasma. K3EDTA human blood plasma was purchased from BioTrend. (D) Stability of TF2D-5 and TF2D-5a in human liver microsomes. Human liver microsomes consisted of 6 mg/mL protein (containing Cytochrome P450 (CYP) enzymes), 75 mM sucrose, 1.3 mM NADPH, 3.3 mM D-glucose-6-phosphat, 0.2 u/mL D-glucose-6-phosphat dehydrogenase, 3.3 mM magnesium chloride, and 100 mM potassium phosphate; pH 7.4.