Figure 14.

Schematic overview of the selection course. Two selections were started in parallel, the target selection 1 (TS1) and the empty selection 1 (ES1). All target selections had the D-tau(271-316) immobilized on the plate surface during selection (green arrow). All empty selections were devoid of the D-tau(271-316) immobilized on the plate surface during selection and were used as negative controls (red arrow). Direct controls (DC2 and DC3) were used as another form of negative control (yellow arrow). Enriched phages of the last target selection round were put onto plates where no D-tau(271-316) had been immobilized and a normal selection round was performed. Negative control sequences were compared with target selection sequences after NGS sequencing to value which peptides were not target-specific.