Table 9.

Conditions for the cell culture of stem cells isolated from mother’s milk.

Source of Cells	Breas Milk (after Isolation by Centrifugation)
Type of cell culture	Adherent (medium volume—10 mL)
Culture vessel	Culture bottle; surface area of the bottom: 25 cm2; TC Flask T25, Cell+; Sarsted; Germany
Culture medium	DMEM (1×) + GlutaMAX™-I [+] 1 g/L D-Glucos [+]; Pyruvate Gibco; UK
Serum	Heat Inactivated FBS; Gibco; USA
Antibiotics	Amphotericin B 250 μg/mL + Penicillin/Streptomycin (100×); PAA; Austria
Temperature	37 °C
O2 concentration	15%
CO2 concentration	5%
Moisture	95%