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. 2023 Jan 24;24(3):2306. doi: 10.3390/ijms24032306

Figure 1.

Figure 1

A time-course study of the expression of ATF1–7 mRNAs following transient ischemia. Representative RT-PCR results of ATF1–7 mRNAs expression in the ipsilateral cortex of rats sub-jected to 60 min of MCA occlusion (A). Three rats (n = 3) for each group and each band represents a data point that was extracted from an independent brain sample and no replicates. The ATF1–7 mRNA bands were quantified and normalized with β-actin mRNA (internal control). The value obtained from the sham-operated controls (sh) was arbitrarily defined as 1 (B). ATF3 and ATF4 mRNAs were the only two isoforms showing significant increase (C). The real time qPCR results of ATF1–7 mRNAs expression in the ipsilateral cortex are shown in panel (D). qPCR were performed in three rats (n = 3), 2 replicates and triplicate for each point. Values: –0.5 and 0 represent 30 and 60 min after the onset of ischemia; 0.5, 1, 1.5, 4, 12, and 24 denote the duration of reperfusion in hours after 60 min of ischemia. Data are expressed as mean ± SD (n ≧ 6). ★ p < 0.05 and ★★ p < 0.01 vs. sh. ATF: activating transcription factor.