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. 2023 Jan 24;24(3):2306. doi: 10.3390/ijms24032306

Figure 3.

Figure 3

ATF3 knockout (KO) exacerbates ischemic brain injury. ATF3 KO mice were subjected to 25 min of ischemia and infract volume was determined at 24 h of reperfusion. Three wild type and three KO mouse brains were used in RT-PCR analysis; and another set of three wild type and three KO mouse brains were used in Western blot analysis. Each band represents a data point that was extracted from an independent brain sample and no replicates. Representative RT-PCR and Western blots of ATF3 mRNA and protein in the ischemic cortices (A). Brain infarct volume in KO and wild type (WT) mice after 25 min of ischemia and 24 h of reperfusion (B) and representative images of brain infarct (C). Each dot represents a data point from an individual animal. Data are expressed as mean ± SD (n ≧ 6). ★★ p < 0.01 vs. control. ATF3: activating transcription factor 3; MCAO: middle cerebral artery occlusion.