Figure 7.

Post-ischemic CTMP siRNA treatment leads to sustained reduction in brain infarct and improves neurological deficit. Rats were subjected to icv infusion of 75 pmol CTMP siRNA right after 35 min of MCA occlusion. Functional outcome based on Bederson’s postural reflex test were measured at 2 and 14 days of reperfusion (A,B). Rats were then subjected to T2-weighted MRI for infarct volume analysis (C), representative T2-weighted MR images of coronal brain sections are shown in panel (D). Each dot represents a data point from an individual animal. Data are expressed as mean ± SD (n ≧ 6). ★ p < 0.05 and ★★ p < 0.01 vs. controls. White area represents infarct area (yellow arrow). (Bars = 1 cm). CTMP: carboxyl-terminal modulator protein; MCAO: middle cerebral artery occlusion; scRNA: scramble RNA; siRNA: small interfering RNA.