Table 1.
Rhythmic parameters (mesor, amplitude and acrophase) for placental macrophages. A p value < 0.05 for mesor, amplitude, and acrophase implies a rhythmicity of the investigated gene.
| Conditions | Gene | Cycle | Mesor | CI 95% | p | Amplitude | CI 95% | p | Acrophase | CI 95% | p |
|---|---|---|---|---|---|---|---|---|---|---|---|
| / | BMAL1 | + | 0.80 | (0.62; 0.98) | <0.001 | 0.56 | (0.31; 0.83) | <0.001 | 16.38 | (14.63; 18.14) | <0.001 |
| − | 0.70 | (0.53; 0.88) | <0.001 | 0.16 | (−0.08; 0,41) | 0.20 | 13.25 | (7.39; 19.11) | 0.67 | ||
| CLOCK | + | 0.36 | (0.16; 0.55) | <0.001 | 0.23 | (−0.04; 0.51) | 0.09 | 17.11 | (12.56; 21.66) | 0.02 | |
| − | 1.21 | (0.66; 1.76) | <0.001 | 0.49 | (−0.2 9; 1.27) | 0.22 | 6.21 | (0.10; 12.32) | 0.06 | ||
| CRY2 | + | 0.73 | (0.50; 0.96) | <0.001 | 0.75 | (0.43; 1.07) | <0.001 | 17.27 | (15.63; 18.87) | <0.001 | |
| − | 0.93 | (0.10; 1.75) | 0.027 | 0.27 | (−0.90; 1.43) | 0.65 | 9.379 | (4.77; 13.99) | 0.75 | ||
| PER2 | + | 6.70 | (4.09; 9.31) | <0.001 | 2.74 | (−0.95; 6.43) | 0.14 | 8.46 | (3.32; 13.60) | 0.18 | |
| − | 1.14 | (0.47; 1.81) | <0.001 | 0.26 | (−0.68; 1.21 | 0.58 | 17.12 | (15.38; 18.86) | 0.46 | ||
| NR1D1 | + | 0.89 | (0.77; 1.01) | <0.001 | 0.85 | (0.68; 1.03) | <0.001 | 16.55 | (15.77; 17.32) | <0.001 | |
| − | 0.87 | (0.32; 1.42) | 0.002 | 0.19 | (−0.58; 0.97) | 0.61 | 15.93 | (13.00; 18.86) | 0.60 | ||
| 4 days of culture | BMAL1 | / | 0.93 | (0.51; 1.35) | <0.001 | 0.38 | (−0.21; 0.97) | 0.21 | 11.87 | (5.88; 17.85) | 0.96 |
| CLOCK | 0.42 | (0.07; 0.77) | 0.01 | 0.03 | (−0.46; 0.53) | 0.89 | 11.69 | / | 0.99 | ||
| CRY2 | 0.87 | (0.42; 1.33) | <0.001 | 0.36 | (−0.27; 0.10) | 0.26 | 11.02 | (4.30; 17.73) | 0.77 | ||
| PER2 | 0.40 | (0.14; 0.66) | 0.003 | 0.28 | (−0.09; 0.65) | 0.13 | 6.28 | (1.28; 11.28) | 0.02 | ||
| NR1D1 | 0.58 | (0.15; 1.01) | 0.008 | 0.22 | (−0.39; 0.82) | 0.48 | 21.43 | / | 0.63 | ||
|
Co-culture with trophoblasts
(4 days) |
BMAL1 | + | 1.58 | (1.23; 1.92) | <0.001 | 0.8 | (0.31; 1.30) | 0.001 | 17.20 | (14.87; 19.55) | <0.001 |
| − | 1.21 | (0.72; 1.71) | <0.001 | 0.14 | (−0.56; 0.84) | 0.70 | 12.99 | (5.39; 20.60) | 0.92 | ||
| CLOCK | + | 1.70 | (0.89; 2.50) | <0.001 | 1.18 | (0.04; 2.33) | 0.04 | 7.36 | (3.68; 11.04) | 0.01 | |
| − | 1.46 | (−0.54; 3.45) | 0.15 | 0.20 | (−2.63; 3.03) | 0.89 | 6.44 | / | 0.84 | ||
| CRY2 | + | 4.69 | (2.47; 6.91) | <0.001 | 1.88 | (−1.26; 5.03) | 0.24 | 11.12 | (4.73; 17.51) | 0.79 | |
| − | 1.38 | (−0.06; 2.82) | 0.06 | 1.04 | (−0.99; 3.07) | 0.32 | 12.31 | (4.83; 19.79) | 0.93 | ||
| PER2 | + | 2.03 | (1.28; 2.79) | <0.001 | 0.93 | (−0.14; 1.99) | 0.09 | 11.58 | (7.18; 15.98) | 0.85 | |
| − | 1.07 | (−0.07; 2.21) | 0.06 | 0.28 | (−1.33; 1.89) | 0.73 | 10.65 | (0.72; 20.58) | 0.90 | ||
| NR1D1 | + | 1.82 | (1.06; 2.59) | <0.001 | 1.16 | (0.07; 2.25) | 0.03 | 6.99 | (3.41; 10.57) | 0.006 | |
| − | 1.48 | (−0.42; 3.38) | 0.12 | 0.27 | (−2.42; 2.96) | 0.12 | 17.57 | / | 0.77 |
+ = significant BMAL1 cycle. − = No significant BMAL1 cycle.