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. 2023 Jan 30;24(3):2583. doi: 10.3390/ijms24032583

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Nuclear localization of ShWRKY81. The constructed plasmid (35S::ShWRKY81-GFP) and control (35S::GFP) in combination with 35S::NLS-dsRED (nuclear marker) were transiently overexpressed in the leaf cells of N. benthamiana via Agrobacterium-mediated transformation. Green and red fluorescence were detected and captured by a laser confocal scanning microscope at 48 h post agroinfiltration. The panels from left to right show GFP images (GFP), dsRED images (DsRed), bright images (Bright), and merged images (Merged), respectively. Bar = 20 µm.