Figure 7.

The silencing of ShWRKY81 reduced the generation of HR in LA1777 following On-Lz infection. Histological observation of HR production at infection sites of On-Lz in control (a) and ShWRKY81-silenced LA1777 seedlings (b). Blue staining (trypan) shows the hypersensitive cell death. The HR production rates were calculated at 24, 48, and 72 hpi (c). Co, conidium; Sh, secondary hyphae; HR, hypersensitive response. Bar, 20 μM. Values are shown as the means ± standard error of three biological replicates, and statistically significant differences are represented by asterisks (Student’s t-test, *, p < 0.05).