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. 2023 Jan 31;24(3):2675. doi: 10.3390/ijms24032675

Figure 7.

Figure 7

Histological evaluations of left ventricle (LV) and right ventricle (RV) from 12-month-old male and female R14del-PLN and WT-PLN mice. (A,C) Representative images of ventricular sections stained with Picrosirius red to evaluate the level of fibrosis by observing collagen deposition in LV and RV tissues from WT-PLN and R14del-PLN in paraffin-embedded sections (4 μm). Picrosirius red staining of LV and RV from R14del-PLN sections showed significant interstitial (black arrows) and perivascular (arrows) deposition of collagen fibers (red) compared with WT-PLN (left panels). Scale bar = 75 μm, 20× magnification views. (B,D) Quantitative analysis of ventricular fibrosis (expressed as a percentage of total area) in WT-PLN and R14del-PLN hearts (right panel). Data are expressed as mean ± SD for the myocyte fibrosis. n = 5 per group.